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Application of propylene glycol in DNA-based studies of invertebrates

by: Weigand, Alexander M.; Desquiotz, Noah; Weigand, Hannah; Szucsich, Nikolaus

Publication date: 2021-1-4
Usage: Attribution 4.0 International
Topics: alternative fixative, bioassessment, DNA integrity, DNA preservation, environmental monitoring
Publisher: Pensoft Publishers
Collection: biodiversity
Contributor: Pensoft Publishers
Language: English
Rights: https://biodiversitylibrary.org/permissions
Rights-holder: Copyright held by individual article author(s).
Volume: 5
Item Size: 31.4M

Abstract: High-throughput sequencing (HTS) studies on invertebrates commonly use ethanol as the main sample fixative (upon collection) and preservative (for storage and curation). However, alternative agents exists, which should not be automatically neglected when studies are newly designed. This review provides an overview of the application of propylene glycol (PG) in DNA-based studies of invertebrates, thus to stimulate an evidence-based discussion.
The use of PG in DNA-based studies of invertebrates is still limited (n = 79), but a steady increase has been visible since 2011. Most studies used PG as a fixative for passive trapping (73%) and performed Sanger sequencing (66%; e.g. DNA barcoding). More recently, HTS setups joined the field (11%). Terrestrial Coleoptera (30%) and Diptera (20%) were the most studied groups. Very often, information on the grade of PG used (75%) or storage conditions (duration, temperature) were lacking. This rendered direct comparisons of study results difficult, and highlight the need for further systematic studies on these subjects.
When compared to absolute ethanol, PG can be more widely and cheaply acquired (e.g. as an antifreeze, 13% of studies). It also enables longer trapping intervals, being especially relevant at remote or hard-to-reach places. Shipping of PG-conserved samples is regarded as risk-free and is authorised, pinpointing its potential for larger trapping programs or citizen science projects. Its property to retain flexibility of morphological characters as well as to lead to a reduced shrinkage effect was especially appraised by integrative study designs. Finally, the so far limited application of PG in the context of HTS showed promising results for short read amplicon sequencing and reduced representation methods. Knowledge of the influence of PG fixation and storage for long(er) read HTS setups is currently unavailable.
Given our review results and taking difficulties of direct methodological comparisons into account, future DNA-based studies of invertebrates should on a case-by-case basis critically scrutinise if the application of PG in their anticipated study design can be of benefit.
Addeddate: 2025-05-08 20:17:05
Bhl_virtual_titleid: 210921
Bhl_virtual_volume: v.5 (2021)
Call number: 10_3897_mbmg_5_57278
Call-number: 10_3897_mbmg_5_57278
Foldoutcount: 0
Genre: article
Identifier: applicationprop5weig
Identifier-ark: ark:/13960/s25mcwcn2bf
Identifier-bib: 10_3897_mbmg_5_57278
Identifier-doi: 10.3897/mbmg.5.57278
Ocr: tesseract 5.3.0-6-g76ae
Ocr_detected_lang: en
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Ocr_detected_script: Latin
Ocr_detected_script_conf: 1.0000
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Pages: 15
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Possible copyright status: In copyright. Digitized with the permission of the rights holder.
Ppi: 300
Source: Metabarcoding and Metagenomics 5
Year: 2021